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Genome-wide identification, expression profiling, and protein-protein interaction properties of ovate family proteins in apple

文献类型： 外文期刊

作者： Li, Huifeng 1 ; Dong, Qinglong 2 ; Zhao, Qiang 3 ; Ran, Kun 1 ;

作者机构： 1.Shandong Inst Pomol, 64 Longtan Rd, Tai An 271000, Shandong, Peoples R China

2.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, 3 Taicheng Rd, Yangling 712100, Shaanxi, Peoples R China

3.Shandong Agr Univ, Coll Hort Sci & Engn, 61 Daizong Rd, Tai An 271000, Shandong, Peoples R China

关键词： Apple; Ovate family protein; Gene cloning; Expression analysis; Protein-protein interaction

期刊名称：TREE GENETICS & GENOMES （ 影响因子：2.297； 五年影响因子：2.547 ）

ISSN： 1614-2942

年卷期： 2019 年 15 卷 3 期

页码：

收录情况： SCI

摘要： As plant-specific transcription factors, ovate family proteins (OFP) were involved in the regulation of plant growth, development, and stress response. Although OFP proteins have been reported in some species, little is known about their evolution, structure, expression levels under biological and abiotic stress, and interactions among OFP members in apple. In this study, 26 apple MdOFP genes were identified. Gene structure analysis showed that the main characteristics of the OFP genes of monocots and dicots were intron-free. Phylogenetic analysis showed that the OFP proteins of monocots and dicots could be divided into 15 subgroups, of which Ia, Ib, Ic, Id, Ie, If, Ig, Ih, Ii, and Ij subgroups were dicots, while IIa, IIb, IIc, IId, and IIe subgroups were monocots. Chromosomal localization analysis showed that 26 MdOFP genes were unevenly distributed on 13 chromosomes. Twelve MdOFP genes were cloned by RT-PCR method and found to be expressed in the tested tissues in different degrees. Under osmotic stress treatment, MdOFP11, MdOFP14, and MdOFP20 responded to NaCl treatment; MdOFP6 and MdOFP14 responded to mannitol treatment. The results of Y2H showed that MdOFP13, MdOFP16, MdOFP20, and MdOFP22 could form heterodimers with multiple MdOFP proteins, while MdOFP16 and MdOFP16 could form homodimers. These results provide valuable references for analyzing the biological function of MdOFP transcription factors in the growth, development, and stress conditions.

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