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Cloning and characterization of a specific UDP-glycosyltransferase gene induced by DON and Fusarium graminearum

文献类型： 外文期刊

作者： Zhao, Lanfei 1 ; Ma, Xin 1 ; Su, Peisen 1 ; Ge, Wenyang 1 ; Wu, Hongyan 2 ; Guo, Xiuxiu 3 ; Li, Anfei 1 ; Wang, Hongwei 1 ;

作者机构： 1.Shandong Agr Univ, Coll Agron, State Key Lab Crop Biol, Shandong Key Lab Crop Biol, 61 Daizong St, Tai An 271018, Shandong, Peoples R China

2.Shandong AgrUnir Fert SciTech Co Ltd, Feicheng 271600, Peoples R China

3.Shandong Acad Agr Sci, Jinan 250100, Shandong, Peoples R China

关键词： UDP-glycosyltransferase; Fusarium graminearum; Triticum aestivum; Deoxynivalenol; TaUGT5

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2018 年 37 卷 4 期

页码：

收录情况： SCI

摘要： TaUGT5: can reduce the proliferation and destruction of F. graminearum and enhance the ability of FHB resistance in wheat. Deoxynivalenol (DON) is one of the most important toxins produced by Fusarium species that enhances the spread of the pathogen in the host. As a defense, the UDP-glycosyltransferase (UGT) family has been deduced to transform DON into the less toxic form DON-3-O-glucoside (D3G), but the specific gene member in wheat that is responsible for Fusarium head blight (FHB) resistance has been little investigated and proved. In this study, a DON and Fusarium graminearum responsive gene TaUGT5, which is specific for resistant cultivars, was cloned with a 1431 bp open reading frame (ORF) encoding 476 amino acids in Sumai3. TaUGT5 is located on chromosome 2B, which has been confirmed in nulli-tetrasomic lines of Chinese Spring (CS) and is solely expressed among three homologs on the A, B and D genomes. Over-expression of this gene in Arabidopsis conferred enhanced tolerance when grown on agar plates that contain DON. Similarly, the coleoptiles of wheat over-expressing TaUGT5 showed more resistance to F. graminearum, evidencing reduced proliferation and destruction of plant tissue by the pathogen. However, the disease resistance in spikes was not as significant as that on coleoptile compared with wild-type plants. A subcellular localization analysis revealed that TaUGT5 was localized on the plasma membrane of tobacco leaf epidermal cells. It is possible that TaUGT5 could enhance tolerance to DON, protect the plant cell from the pathogen infection and result in better maintenance of the cell structure, which slows down pathogen proliferation in plant tissue.

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