文献类型： 外文期刊
作者： Li, Yueyue 1 ; Tan, Guanlin 2 ; Lan, Pingxiu 1 ; Zhang, Ansheng 3 ; Liu, Yong 4 ; Li, Ruhui 5 ; Li, Fan 1 ;
作者机构： 1.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Y, Kunming 650201, Yunnan, Peoples R China
2.Yunnan Agr Univ, Modern Educ Technol Ctr, Kunming 650201, Yunnan, Peoples R China
3.Shandong Acad Agr Sci, Inst Plant Protect, Jinan 250100, Shandong, Peoples R China
4.Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China
5.USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA
关键词： Tobamovirus; Universal primers; Generic RT-PCR assay; Virus detection
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）
ISSN： 0166-0934
年卷期： 2018 年 259 卷
页码：
收录情况： SCI
摘要： A generic RT-PCR assay was developed for the universal detection of viruses of the genus Tobamovirus using a novel pair of degenerate primers designed based on conserved regions on replicase genes of 32 tobamoviruses. The assay detected nine tobamoviruses, including six Solanaceae-infecting subgroup tobamoviruses of Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV), Tomato mottle mosaic virus (ToMMV), Tobacco mottle green mosaic virus (TMGMV), Pepper mild mottle virus (PMMoV), Paprika mild mottle virus (PaMMV), one Orchidaceae-infecting tobamovirus of Odontoglossum ringspot virus (ORSV) and two Cucurbitaceae-infecting subgroup tobamoviruses of Cucumber green mottle mosaic virus (CGMMV) and Zucchini green mottle mosaic virus (ZGMMV), with high amplification efficiency, specificity and sensitivity. The assay was applied to detect tobamoviruses in pepper and tomato fields. Five tobamoviruses, PMMoV, TMV, ToMV, ToMMV and TMGMV, were detected from the pepper fields in single and mixed infections. Single infections of PMMoV, ToMV and ToMMV and mix-infection of ToMV + PMMoV were detected from the tomato fields. Among these viruses, PMMoV was first detected from tomato worldwide, while ToMMV was first detected from tomato plants in China. This generic assay is simple, cost-effective and has great potential to detect more tobamoviruses in the field.
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