文献类型： 外文期刊
作者： Li, Fan 1 ; Sun, Hongwei 1 ; Yang, Shuke 1 ; Gao, Rui 1 ; Xu, Xiao Hui 1 ; Lu, Xingbo 1 ;
作者机构： 1.Shandong Acad Agr Sci, Shandong Key Lab Plant Virol, Inst Plant Protect, Jinan, Shandong, Peoples R China
关键词： bias; lanthanide ions; polymerase chain reaction; preference
期刊名称：BIOTECHNIQUES （ 影响因子：1.993； 五年影响因子：2.541 ）
ISSN： 0736-6205
年卷期： 2018 年 65 卷 1 期
页码：
收录情况： SCI
摘要： Rare earth elements have many uses, and are frequently included in products such as fluorescent materials, hydride batteries, catalytic materials and lasers. In this study, it was observed that trivalent lanthanide ions (Ln[III] ions) appeared to inhibit the synthesis of large fragments in PCR assays, thus resulting in the preferential amplification of shorter sequences. It is therefore speculated that this Ln(III) ion-mediated bias could be utilized to improve the success rates for amplification of shorter products.
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