Purification, characterization, and determination of biological activities of water-soluble polysaccharides from Mahonia bealei
文献类型： 外文期刊
作者： Kakar, Mohib Ullah 1 ; Li, Jingyi 1 ; Mehboob, Muhammad Zubair 3 ; Sami, Rokayya 4 ; Benajiba, Nada 5 ; Ahmed, Aziz 2 ; Nazir, Amina 6 ; Deng, Yulin 1 ; Li, Bo 1 ; Dai, Rongji 1 ;
作者机构： 1.Beijing Inst Technol BIT, Sch Life Sci, Beijing Key Lab Separat & Anal Biomed & Pharmaceu, Beijing 100081, Peoples R China
2.Lasbela Univ Agr Water & Marine Sci, LUAWMS, Fac Marine Sci, Uthal, Balochistan, Pakistan
3.Univ Chinese Acad Sci, Coll Life Sci, CAS Ctr Excellence Biot Interact, Beijing 100049, Peoples R China
4.Taif Univ, Coll Sci, Dept Food Sci & Nutr, PO 11099, Taif 21944, Saudi Arabia
5.Princess Nourah bint Abdulrahman Univ, Dept Basic Hlth Sci, POB 84428, Riyadh 11671, Saudi Arabia
6.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Ind North Rd 202, Jinan 202, Shandong, Peoples R China
7.Beijing Inst Technol, Adv Res Inst Multidisciplinary Sci, Beijing 100081, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.996； 五年影响因子：5.516 ）
ISSN： 2045-2322
年卷期： 2022 年 12 卷 1 期
页码：
收录情况： SCI
摘要： Mahonia bealei is one of the important members of the genus Mahonia and Traditional Chinese Medicine (TCM). Several compounds isolated from this plant have exhibited useful biological activities. Polysaccharides, an important biomacromolecule have been underexplored in case of M. bealei. In this study, hot water extraction and ethanol precipitation were used for the extraction of polysaccharides from the stem of M. bealei, and then extract was purified using ultrafiltration membrane at 50,000 Da cut off value. Characterization of the purified M. bealei polysaccharide (MBP) was performed using Fourier Transform Infrared Spectroscopy (FT-IR), along with Scanning Electron Microscopy (SEM), X-ray crystallography XRD analysis and Thermal gravimetric analysis (TGA). The purified polysaccharide MBP was tested for antioxidant potential by determining its reducing power, besides determining the DPPH, ABTS, superoxide radical, and hydroxyl radical scavenging along with ferrous ion chelating activities. An increased antioxidant activity of the polysaccharide was reported with increase in concentration (0.5 to 5 mg/ml) for all the parameters. Antimicrobial potential was determined against gram positive and gram-negative bacteria. 20 mu g/ml MBP was found appropriate with 12 h incubation period against Escherichia coli and Bacillus subtilis bacteria. We conclude that polysaccharides from M. bealei possess potential ability of biological importance; however, more studies are required for elucidation of their structure and useful activities.
- 相关文献
作者其他论文 更多>>
-
Effects of Gibberellic Acid on Soluble Sugar Content, Organic Acid Composition, Endogenous Hormone Levels, and Carbon Sink Strength in Shine Muscat Grapes during Berry Development Stage
作者：Li, Xiujie;Wu, Yusen;Han, Zhen;Yang, Guowei;Liu, Li;Li, Bo;Cai, Zhonghui;Xie, Zhaosen;Liu, Xueli;Li, Shaoxuan
关键词：gibberellin; grape; soluble sugar; organic acid; sugar unloading; sink strength
-
Alkaline Invertase 2 positively modulates cold adaptive of Camellia sinensis and enhances freezing and salt tolerance in transgenic Arabidopsis thaliana
作者：Li, Bo;Wang, Huan;He, Shan;Liang, Shicai;Wang, Yu;Fan, Kai;Qian, Wenjun;Song, Chuankui;Li, Bo;Wang, Huan;He, Shan;Liang, Shicai;Wang, Yu;Fan, Kai;Qian, Wenjun;Zhang, Shuning;Yamashita, Hiroto;Ikka, Takashi;Ding, Zhaotang
关键词：Camellia sinensis; Alkaline/neutral invertase; CsINV2; Arabidopsis; Cold; Salt
-
Characterization, taxonomic classification, and genomic analysis of two newly isolated bacteriophages with potential to infect Escherichia coli
作者：Nazir, Amina;Li, Lulu;Liu, Yuqing;Chen, Yibao;Nazir, Amina;Li, Lulu;Liu, Yuqing;Chen, Yibao;Nazir, Amina;Li, Fei;Tong, Yigang
关键词：Escherichia coli; bacteriophage; antibiotics; kayfunavirus genus; taxonomy and genomic
-
Effects of GA3 Treatments on Fruit Vascular Structure and Water Transport of Grape
作者：Cai, Zhong-Hui;Wang, Yue;Xie, Zhao-Sen;Li, Xiu-Jie;Li, Bo;Forney, Charles F.
关键词：Grape; GA(3); vascular bundle; water transport; sugar unloading
-
A Simple Clear Technique in Observing Vascular Development of Grape Ovary
作者：Fei, Teng;Li, Youmei;Xie, Zhaosen;Li, Bo
关键词：Vitaceae; microscopy; xylem
-
Observation of Flowering Process of Grape (Vitis vinifera L.)-Insight into the Starting of Pollination of Flower Hiding in Calyptras (Cap)
作者：Wang, Yue;Cai, Zhonghui;Xie, Zhaosen;Li, Xiujie;Wu, Yusen;Li, Bo
关键词：Cleistogamy; pollen structure; stigma receptivity; pollen tube germination
-
Isolation, characterization and genomic analysis of a novel phage IME178 with lytic activity against Escherichia coli
作者：Li, Fei;Na, Shi;Zhang, Deqing;Li, Lu;Zhao, Jian;Liu, Fei;Liu, Pengfei;Li, Yuexiu;Li, Ming;Lei, Ming;Nazir, Amina;Wang, Guojun
关键词：Phage; Multi -drug resistance; Escherichia coli; Demerecviridae; Nosocomial infections
