Activating pathway of three metabolic detoxification phases via down-regulated endogenous microRNAs, modulates triflumezopyrim tolerance in the small brown planthopper, Laodelphax striatellus (Fallen)
文献类型： 外文期刊
作者： Yang, Yuanxue 1 ; Wang, Aiyu 1 ; Zhang, Yun 1 ; Xue, Chao 1 ; Zhao, Ming 1 ; Zhang, Jianhua 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Ind Crops, Jinan, Peoples R China
关键词： Metabolic detoxification phases; Insecticide tolerance; Regulatory mechanism
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）
ISSN： 0141-8130
年卷期： 2022 年 222 卷
页码：
收录情况： SCI
摘要： Frequent use of insecticide causes an environmental hazard, and also leads pest to develop insecticide resistance. Enhancement of metabolic detoxification and reduction of target sensitivity are the primary mechanism of insecticide resistance. Clarifying the regulatory pathway of resistance mechanism states a pivotal theoretical foundation of delaying insecticide resistance development. Here, we show that three endogenous microRNAs, PC-3p-2522_840, PC-3p-446_6601 and PC-5p-3096_674, are required for the small brown planthopper (SBPH) to modulate triflumezopyrim tolerance via activating pathways of three metabolic detoxification phases. Twenty-one down-regulated miRNAs were acquired, and PC-5p-3096_674, PC-3p-446_6601 and PC-3p-2522_840 were the three most significantly down-regulated miRNAs during triflumezopyrim exposure. The mortality of SBPH was significantly increased after over-supplementation of PC-5p-3096_674, PC-3p-446_6601 and PC -3p-2522_840, with triflumezopyrim exposure. Moreover, the interactions between PC-3p-2522_840 and cyto-chrome P450 CYP6FL1, PC-3p-446_6601 and glutathione S-transferase GSTD2, UDP-Glycosyltransferase UGT386F1, PC-5p-3096_674 and ATP-binding cassette transporters ABCA3 were systematically demonstrated through the dual luciferase reporter assay. Besides, the mortality of SBPH was significantly increased after knockdown of CYP6FL1, GSTD2, UGT386F1 and ABCA3 with triflumezopyrim exposure. These findings uncover a strategy whereby the SBPH weakens three endogenous microRNAs to activate pathways of three metabolic detoxification phases via targeting CYP6FL1, GSTD2, UGT386F1 and ABCA3 and promotes its tolerance to triflumezopyrim.
- 相关文献
作者其他论文 更多>>
-
Anti-Tumor Activity and Mechanism of Silibinin Based on Network Pharmacology and Experimental Verification
作者：Li, Peihai;Wang, Dexu;Yang, Xueliang;Liu, Changyu;Li, Xiaobin;Zhang, Xuanming;Liu, Kechun;Zhang, Yun;Wang, Rongchun;Zhang, Mengqi;Wang, Changyun
关键词：silibinin; anti-tumor; network pharmacology; adenoid cystic carcinoma; Western blot analysis
-
Molecular mechanisms of cytochrome P450-mediated detoxification of tetraniliprole, spinetoram, and emamectin benzoate in the fall armyworm, Spodoptera frugiperda (JE Smith)
作者：Wang, Aiyu;Zhang, Yun;Xue, Chao;Zhao, Ming;Yang, Yuanxue;Zhang, Jianhua;Wang, Aiyu;Zhang, Yun;Zhao, Ming;Yang, Yuanxue;Zhang, Jianhua;Liu, Shaofang;Zhao, Yongxin
关键词：enzyme activity; insecticide; P450 genes; RNA interference; Spodoptera frugiperda; synergistic effect
-
Enantioselective Synthesis of the Active Sex Pheromone Components of the Female Lichen Moth, Lyclene dharma dharma, and Their Enantiomers
作者：Zhou, Yun;Zhang, Yueru;Fu, Xiaochen;Xie, Hongqing;Han, Jinlong;Zhang, Jianhua;Shan, Chenggang;Wang, Jianan;Zhong, Jiangchun
关键词：sex pheromone; Lyclene dharma dharma; Evans' chiral auxiliary; enantioselective synthesis
-
Transcriptome reveals the molecular mechanism of chaetoviridin A inhibiting the spore germination of Verticillium dahliae
作者：Zhang, Yun;Yang, Yuanxue;Wang, Aiyu;Xue, Chao;Zhao, Ming;Zhang, Jianhua
关键词：Chaetoviridin A; Verticillium dahliae; Spore germination; Molecular mechanism; Transcriptome
-
MicroRNA PC-5p-3991_515 mediates triflumezopyrim susceptibility in the small brown planthopper through regulating the post-transcriptional expression of P450 CYP417A2
作者：Yang, Yuanxue;Wang, Aiyu;Xue, Chao;Zhang, Yun;Zhao, Ming;Zhang, Jianhua;Yang, Yuanxue;Liu, Zewen;Tian, Honglin;Zhou, Maolin;Liu, Zewen;Zhang, Jianhua
关键词：Laodelphax striatellus; microRNA PC-5p-3991_515; triflumezopyrim
-
Azotobacter inoculation can enhance the resistance of Bt cotton to cotton bollworm, Helicoverpa armigera
作者：Li, Zhuo;Li, Lei;Yuan, Yi-Yang;Ge, Feng;Li, Zhuo;Zhao, Ming;Chen, Fa-Jun;Parajulee, Megha N.;Ge, Feng
关键词：Azotobacter; Bt protein expression; development and fecundity; food utilization; nitrogen metabolism-related compounds; transgenic Bt cotton
-
A microRNA, PC-5p-30_205949, regulates triflumezopyrim susceptibility in Laodelphax striatellus (Fallen) by targeting CYP419A1 and ABCG23
作者：Wang, Aiyu;Yang, Yuanxue;Zhou, Yun;Zhang, Yun;Xue, Chao;Zhao, Ming;Zhang, Jianhua;Wang, Aiyu;Yang, Yuanxue;Zhou, Yun;Zhang, Yun;Xue, Chao;Zhao, Ming;Zhang, Jianhua;Zhao, Yongxin
关键词：Laodelphax striatellus; Triflumezopyrim; PC-5p-30_205949; ABCG23
