文献类型： 外文期刊

作者： Pang, QX ¹ ; Zhang, SC ² ; Wang, CF ² ; Shi, XD ² ; Sun, YN ² ;

作者机构： 1.Ocean Univ China, Dept Marine Biol, Qingdao 266003, Peoples R China

2.Ocean Univ China, Dept Marine Biol, Qingdao 266003, Peoples R China; Shandong Univ, Shandong Acad Agr Sci, Jinan 250100, Shandong Prov, Peoples R China

关键词： Amphioxus;Branchiostoma belcheri tsingtauense;Phenoloxidase;Silkworm bombyx-mori;Crayfish blood-cells;Activating system;Phenoloxidase activity;Manduca-sexta;Lucilia-cuprina;Phenol oxidase;Marine mussel;Hemolymph;Purification

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

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收录情况： SCI

摘要： The presence of phenoloxidase (PO) activity in the humoral fluid of amphioxus Branchiostoma belcheri tsingtauense was electrophoretically and spectrophotometrically studied. The enzyme was present in the Immoral fluid predominantly as an inactive proenzyme, prophenoloxidase (proPO). The optimum temperature for activation of the proPO ranged from 30 degreesC to 35 degreesC, and the enzyme exhibited optimum activity at pH between 7.0 and 7.5. ProPO in the humoral fluid was readily activated to active form PO by exogenous elicitors such as trypsin, zymosan and LPS. The activation of the proPO by exogenous elicitors was significantly enhanced in the presence of 10 mM Ca2+, but was susceptible to serine protease inhibitors like soybean trypsin inhibitor and p-nitrophenyt-p'-guanidinobenzoate. PAGE revealed a single band of PO activity in the humoral fluid with an apparent molecular mass of 150 kDa, which was resolved to three bands with molecular masses of 44, 46 and 72 kDa, respectively, after SDS-PAGE. This is the first report on the presence of the enzyme PO in amphioxus humoral fluid.