Expression Pattern of RpCSP6 from Rhopalosiphum padi and Its Binding Mechanism with Deltamethrin: Insights into Chemosensory Protein-Mediated Insecticide Resistance
文献类型： 外文期刊
作者： Gao, Ping 1 ; Tan, Junjie 1 ; Peng, Xiong 1 ; Song, Yue 1 ; Qu, Mingjing 2 ; Chen, Maohua 1 ;
作者机构： 1.Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Minist Educ,Key Lab Plant Protect Resources & Pest, Yangling 712100, Shaanxi, Peoples R China
2.Shandong Acad Agr Sci, Shandong Peanut Res Inst, Qingdao 266100, Shandong, Peoples R China
关键词： the bird cherry-oataphid; chemosensory protein; deltamethrin; fluorescence competitive binding assay; site-directed mutagenesis
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.7； 五年影响因子：6.0 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 32 期
页码：
收录情况： SCI
摘要： The mechanisms of insecticide resistance are complex. Recent studies have revealed a novel mechanism involving the chemosensory system in insecticide resistance. However, the specific binding mechanism between olfactory-related genes and insecticides needs to be clarified. In this study, the binding mechanism between pyrethroid insecticide deltamethrin and RpCSP6 from Rhopalosiphum padi was investigated by using computational and multiple experimental methods. RpCSP6 was expressed in different tissues and developmental stages of R. padi and can be induced by deltamethrin. Knockdown of RpCSP6 significantly increased the susceptibility of R. padi to deltamethrin. The binding affinity of RpCSP6 to 24 commonly used insecticides was measured. Seven key residues were found to steadily interact with deltamethrin, indicating their significance in the binding affinity to the insecticide. Our research provided insights for effectively analyzing the binding mechanism of insect CSPs with insecticides, facilitating the development of new and effective insecticides that target insect CSPs.
- 相关文献
作者其他论文 更多>>
-
Key residues involved in the interaction between chlorpyrifos and a chemosensory protein in Rhopalosiphum padi : Implication for tracking chemical residues via insect olfactory proteins
作者：Gao, Ping;Tan, Junjie;Peng, Xiong;Chen, Maohua;Qu, Mingjing
关键词：CSP; Chlorpyrifos; Fluorescence competitive binding assay; Binding affinity
-
Identification of purple nutsedge (Cyperus rotundus L.) ecotypes and the effect of environmental factors on tuber sprouting in China
作者：Du, Long;Qu, Chunjuan;Shi, Chengren;Jiang, Xiaojing;Li, Xiao;Ju, Qian;Qu, Mingjing;Gao, Xingxiang;Bai, Shuang
关键词：Cyperus rotundus L; ecotype; genetic diversity; tuber sprouting
-
Coding of Non-coding RNA: Insights Into the Regulatory Functions of Pri-MicroRNA-Encoded Peptides in Plants
作者：Ren, Yi;Song, Yue;He, Juan;Wang, Lei;Song, Shiren;Xu, Wenping;Zhang, Caixi;Ma, Chao;Wang, Shiping;Zhang, Lipeng;Guo, Dinghan;Lers, Amnon;Wang, Shiping
关键词：miPEP; miRNA-encoded peptide; miRNA; pri-miRNA; non-coding RNA; peptides
-
Structure-Based Optimization of N-Substituted Oseltamivir Derivatives as Potent Anti-Influenza A Virus Agents with Significantly Improved Potency against Oseltamivir-Resistant N1-H274Y Variant
作者：Zhang, Jian;Tian, Ye;Fang, Zengjun;Kang, Dongwei;Kong, Xiujie;Jia, Haiyong;Sun, Zhuosen;Jia, Ruifang;Gao, Ping;Xu, Wenfang;Ding, Xiao;Zhan, Peng;Liu, Xinyong;Murugan, Natarajan Arul;Tian, Ye;Bertagnin, Chiara;Loregian, Arianna;Ma, Xiuli;Huang, Bing;Poongayanam, Vasanthanathan;Fang, Zengjun;Poongayanam, Vasanthanathan
关键词：
-
Optimization of N-Substituted Oseltamivir Derivatives as Potent Inhibitors of Group-1 and-2 Influenza A Neuraminidases, Including a Drug-Resistant Variant
作者：Zhang, Jian;Kang, Dongwei;Kong, Xiujie;Ju, Han;Lu, Xueyi;Gao, Ping;Tian, Ye;Jia, Haiyong;Desta, Samuel;Ding, Xiao;Sun, Lin;Huang, Boshi;Liang, Xuewu;Jia, Ruifang;Xu, Wenfang;Zhan, Peng;Liu, Xinyong;Poongavanam, Vasanthanathan;Lu, Huamei;Ma, Xiuli;Huang, Bing;Bertagnin, Chiara;Loregian, Arianna;Murugan, Natarajan Arul;Fang, Zengjun;Poongavanam, Vasanthanathan
关键词：
-
Involvement of chemosensory proteins in host plant searching in the bird cherry-oat aphid
作者：Peng, Xiong;Liu, Lang;Huang, Yi-Xiao;Wang, Su-Ji;Li, De-Xian;Chen, Shu-Ting;Chen, Mao-Hua;Simon, Jean-Christophe;Qu, Ming-Jing
关键词：host choice experiment; olfactometer; Rhopalosiphum padi; RNAi; starvation; wing form
