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Small RNA Sequencing Analysis of miRNA Expression Reveals Novel Insihts into Root Formation under Root Restriction Cultivation in Grapevine (Vitis vinifera L.)

文献类型： 外文期刊

作者： Li, Hui 1 ; Gao, Zhen 2 ; Zahid, Muhammad Salman 1 ; Li, Dongmei 1 ; Javed, Hafiz Umer 1 ; Wang, Lei 1 ; Song, Shiren 1 ; Zhao, Liping 1 ; Xu, Wenping 1 ; Zhang, Caixi 1 ; Ma, Chao 1 ; Wang, Shiping 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Plant Sci, Shanghai 200240, Peoples R China

2.Shandong Agr Univ, Coll Hort Sci & Engn, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

3.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Key Lab Agroprod Proc Technol Shandong, Jinan 250100, Peoples R China

关键词： microRNA; Vitis vinifera; root restriction; root architecture; high-throughput sequencing

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 10 期

页码：

收录情况： SCI

摘要： Root restriction cultivation (RRC) can influence plant root architecture, but its root phenotypic changes and molecular mechanisms are still unknown. In this study, phenotype observations of grapevine root under RRC and control cultivation (nRC) at 12 time points were conducted, and the root phenotype showed an increase of adventitious and lateral root numbers and root tip degeneration after RRC cultivation from 70 days after planting (DAP). The 70 and 125 DAP sampling of two different cultivations, named nR70, RR70, nR125, and RR125, were selected for small RNA sequencing. A total of 153 known miRNAs and 119 predicted novel miRNAs were obtained. Furthermore, BLAST was used to predict the novel miRNAs with miRBase databases using the default parameters; 96 of the 119 predicted novel miRNAs were similar to other species, and the remaining 23 grapevine-specific novel miRNAs were obtained. There were 26, 33, 26, and 32 miRNAs that were differentially expressed in different comparison groups (RR70 vs. nR70, RR125 vs. nR125, nR125 vs. nR70 and RR125 vs. RR70). Target genes prediction of differentially expressed miRNAs was annotated on a variety of biological processes, and 24 participated in root development. Moreover, multiple miRNAs were found to jointly regulate lateral root development under root restriction conditions. The miRNA expression pattern comparison between RRC and nRC may provide a framework for the future analysis of miRNAs associated with root development in grapevine.

  • 相关文献

[1]Small RNA Sequencing Analysis of miRNA Expression Reveals Novel Insihts into Root Formation under Root Restriction Cultivation in Grapevine (Vitis vinifera L.). Li, Hui,Zahid, Muhammad Salman,Li, Dongmei,Javed, Hafiz Umer,Wang, Lei,Song, Shiren,Zhao, Liping,Xu, Wenping,Zhang, Caixi,Ma, Chao,Wang, Shiping,Gao, Zhen,Wang, Shiping. 2020

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