Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity
文献类型： 外文期刊
作者： Zhang, C. 1 ; Song, Y. 1 ; Jiang, F. 1 ; Li, G. 2 ; Jiang, Y. 1 ; Zhu, C. 1 ; Wen, F. 1 ;
作者机构： 1.Shandong Agr Univ, State Key Lab Crop Biol, Shandong Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
2.Shandong Acad Agr Sci, Rice Res Inst, Jinan 272100, Shandong, Peoples R China
关键词： Agrobacterium tumefaciens; Nicotiana tabacum; Oryza sativa; Potato virus Y; Rice stripe virus
期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）
ISSN： 0006-3134
年卷期： 2012 年 56 卷 4 期
页码：
收录情况： SCI
摘要： To induce virus resistance in tobacco and rice we constructed hairpin RNA expression system harbouring inverted repeat fragments of coat protein cDNA of Potato virus Y (PVY) or Rice stripe virus (RSV). These structures were driven by three promoters [cauliflower mosaic virus 35S (CaMV 35S), polyubiqutin gene of maize (Ubi), and Pharbitis nil leucine zipper gene (PNZIP)] which have different tissue-specific activity. PVY resistance ratios were 65.18, 24.33 and 83.54 % in transgenic tobacco plants harboring p35S-PVY, pUbi-PVY and pPNZIP-PVY. RSV resistance was 16.21, 28.61 and 29.33 % in transgenic rice plants harboring p35S-RSV, pUbi-RSV and pPNZIP-RSV. Northern blotting and GUS assay demonstrated that virus resistance levels were related to promoter activity. Therefore, choice of the more effective and tissue-specific promoter to reinforce transcription of hpRNAs will favour the cultivation of highly virusresistant transgenic plants.
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