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Peanut violaxanthin de-epoxidase alleviates the sensitivity of PSII photoinhibition to heat and high irradiance stress in transgenic tobacco

文献类型： 外文期刊

作者： Yang, Sha 1 ; Meng, De-Yun 1 ; Hou, Lin-Lin 1 ; Li, Yan 1 ; Guo, Feng 1 ; Meng, Jing-Jing 1 ; Wan, Shu-Bo 2 ; Li, Xin-Guo 1 ;

作者机构： 1.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Peoples R China

2.Shandong Acad Agr Sci, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan 250100, Peoples R China

3.Shandong Univ, Coll Life Sci, Jinan 250100, Peoples R China

4.QingDao Agr Univ, Agron & Plant Protect Coll, Qingdao 266109, Peoples R China

关键词： Heat and high light stress; Peanut (Arachis hypogaea L.); Photoinhibition; Transgenic tobacco; Violaxanthin de-epoxidase

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2015 年 34 卷 8 期

页码：

收录情况： SCI

摘要： This is the first study on peanut VDE, which led to multiple biochemical and physiological changes to heat and HI stress by improving de-epoxidation of the xanthophylls cycle. A peanut (Arachis hypogaea L.) violaxanthin de-epoxidase gene (AhVDE) was isolated by RT-PCR and RACE methods. The deduced amino acid sequence of AhVDE showed high identities with violaxanthin de-epoxidase of other plant species. The expression of AhVDE was obviously upregulated by 4, 40 A degrees C and high light, NaCl, and abscisic acid. Sense and RNAi transgenic tobaccos were further used to investigate the physiological effects and functional mechanism of AhVDE. Compared with WT, the content of Z, the ratio of (A + Z)/(V + A + Z) and the non-photochemical quenching were higher in sense plants, and lower in the RNAi lines under heat and high irradiance (HI) stress, respectively. Additionally, photoinhibition of photosystem II (PSII) reflected by the maximal photochemical efficiency in WT lines was more severe, and in the RNAi lines was the most severe compared with that in the sense lines. Meanwhile, overexpressing AhVDE also led to multiple biochemical and physiological changes under heat and HI stress. Higher activities of superoxide dismutase and ascorbate peroxidase, lower content of reactive oxygen species and slighter membrane damage were observed in sense lines after heat and HI stress. These results suggested that, peanut VDE can alleviate PSII photoinhibition to heat and HI stress by improving the xanthophyll cycle-dependent energy dissipation.

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