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An RNA-Seq analysis of the peach transcriptome with a focus on genes associated with skin colour

文献类型： 外文期刊

作者： He, Ping 1 ; Li, Linguang 1 ; Wang, Haibo 1 ; Chang, Yuansheng 1 ;

作者机构： 1.Shandong Inst Pomol, Tai An, Shandong, Peoples R China

关键词： anthocyanin; pericarp colour; Prunus persica; sequencing

期刊名称：CZECH JOURNAL OF GENETICS AND PLANT BREEDING （ 影响因子：0.865； 五年影响因子：1.017 ）

ISSN： 1212-1975

年卷期： 2019 年 55 卷 4 期

页码：

收录情况： SCI

摘要： Red pigmentation of the skin is an important consumer trait in peach (Prunus persica). The pigment consists largely of anthocyanin. Here, a transcriptomic contrast, based on RNA-Seq technology, was drawn between a white-skinned (Feitao) and a red-skinned (Qiuxue) peach cultivar. The analysis identified 2407 genes as differentially transcribed in the fruit skin of the two cultivars. Among these were a number of genes known to contribute to anthocyanin synthesis. A quantitative real-time PCR assay was used to validate the RNA-Seq-based estimates of transcript abundance for 14 differentially transcribed genes. Anthocyanin synthesis was observed in the skin of Qiuxue fruit during the late ripening stage, matching the high transcript abundance of the gene encoding UDP glucose: flavonoid 3-O-glucosyltransferase, the final step in the synthesis of anthocyanin.

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