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Effects of Wnt10b on dermal papilla cells via the canonical Wnt/beta-catenin signalling pathway in the Angora rabbit

文献类型： 外文期刊

作者： Bai, Liya 1 ; Gao, Shuxia 1 ; Sun, Haitao 1 ; Zhao, Xueyan 1 ; Yang, Liping 1 ; Hu, Hongmei 1 ; Sun, Jie 2 ; Jiang, Wenxu 1 ;

作者机构： 1.Shandong Acad Agr Sci, Shandong Prov Key Lab Anim Dis Control & Breeding, Inst Anim Sci & Vet Med, Jinan, Shandong, Peoples R China

2.Xingtai Bur Agr & Rural Affairs, Xingtai, Peoples R China

关键词： angora rabbit; dermal papilla cells; Wnt; beta-catenin signalling pathway; Wnt10b; beta-catenin

期刊名称：JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION （ 影响因子：2.13； 五年影响因子：2.322 ）

ISSN： 0931-2439

年卷期： 2019 年 103 卷 5 期

页码：

收录情况： SCI

摘要： Wnt10b is a member of Wnt family that plays a variety of roles in biological functions, including those in the development of hair follicles. To investigate the effect of Wnt10b on hair growth in the Angora rabbit and to determine the underlying molecular mechanism, we cultured dermal papilla (DP) cells with exogenous Wnt10b in vitro. We observed the expressions of downstream critical gene beta-catenin and lymphoid enhancer-binding factor 1 (LEF1) in Wnt/beta-catenin pathway. The levels of beta-catenin mRNA and protein were higher in the Wnt10b group of DP cells than in the Control group, and the mRNA level of LEF1 in the Wnt10b group was higher than in the Control group. Moreover, translocation of beta-catenin from cytoplasm to nucleus was activated in the Wnt10b group. Furthermore, the mRNA levels of the hair follicle-regulatory genes, insulin-like growth factor-1 (IGF-1) and alkaline phosphatase (ALP), and the protein activity of ALP was also upregulated in the Wnt10b group compared to their corresponding levels in the Control group. These data suggest that Wnt10b could activate the canonical Wnt/beta-catenin signalling pathway to induce DP cells in the Angora rabbit. In addition, the proliferation of DP cells was significantly promoted when cultured with Wnt10b for 48 and 72 hr, suggesting that Wnt10b plays a pivotal role in the proliferation and maintenance of DP cells in vitro. In conclusion, this study demonstrates that Wnt10b may promote hair follicle growth in Angora rabbit through the canonical Wnt/beta-catenin signalling pathway that promotes the proliferation of DP cells.

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