Comprehensive transcriptomics and proteomics analyses of rice stripe virus-resistant transgenic rice
文献类型： 外文期刊
作者： Xu, Yang 1 ; Bi, Lvjie 1 ; Yu, Zipeng 1 ; Lin, Chao 3 ; Gan, Liming 1 ; Zhu, Lifei 1 ; Li, Haiyang 1 ; Song, Yunzhi 1 ; Zhu, 1 ;
作者机构： 1.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
2.Shandong Acad Agr Sci, Shandong Peanut Res Inst, Qingdao 266100, Shandong, Peoples R China
3.ShanDong Entry Exit Inspect & Quarantine Bur, Qingdao 266500, Shandong, Peoples R China
关键词： Biosafety assessment; Oryza sativa; proteomics; rice stripe virus; transgenic rice; transcriptomics; virus resistance
期刊名称：JOURNAL OF BIOSCIENCES （ 影响因子：1.826； 五年影响因子：2.107 ）
ISSN： 0250-5991
年卷期： 2019 年 44 卷 4 期
页码：
收录情况： SCI
摘要： Stable transgenic rice line (named KRSV-1) with strong resistance against rice stripe virus was generated using the gene sequence of disease-specific protein by RNA interference. Comprehensive safety assessment of transgenic plants has turned into a significant field of genetic modification food safety. In this study, a safety assessment of KRSV-1 was carried out in a stepwise approach. The molecular analysis exhibited that KRSV-1 harbored one copy number of transgene, which was integrated into the intergenic non-coding region of chromosome 2 associated with inter-chromosomal translocations of 1.6-kb segments of chromosome 8. Then, transcriptomics and proteomics analyses were carried out to detect the unintended effects as a result of the integration of the transgene. Although 650 dramatically differentially expressed genes (DDEGs) and 357 differentially expressed proteins were detected between KRSV-1 and wild-type (WT) by transcriptomics and proteomics analyses, no harmful members in the form of toxic proteins and allergens were observed. Encouragingly, the nutritional compositions of seeds from KRSV-1 were comparable with WT seeds. The results of this entire study of molecular analysis, transcriptome and proteome profile of KRSV-1 revealed that no detrimental changes in the form of toxic proteins and allergens were detected in the transgenic rice line due to the integration of the transgene.
- 相关文献
作者其他论文 更多>>
-
Early detection of gray blight in tea leaves and rapid screening of resistance varieties by hyperspectral imaging technology
作者：Mao, Yilin;Li, He;Wang, Shuangshuang;Ding, Zhaotang;Xu, Yang;Yin, Xinyue;Fan, Kai;Ding, Zhaotang;Wang, Yu
关键词：tea plants; gray blight; hyperspectral; deep learning; disease resistance
-
A Novel Strategy for Constructing Ecological Index of Tea Plantations Integrating Remote Sensing and Environmental Data
作者：Mao, Yilin;Li, He;Shen, Jiazhi;Ding, Zhaotang;Sun, Litao;Wang, Yu;Xu, Yang;Fan, Kai;Han, Xiao;Ma, Qingping;Shi, Hongtao;Bi, Caihong;Feng, Yunlai
关键词：Plantations; Monitoring; Remote sensing; Temperature sensors; Temperature measurement; Ecosystems; Humidity; Convolutional neural networks gate recurrent unit (CNN-GRU); ecological tea plantation; environmental parameters; multisource remote sensing; plant community; UAV
-
A Magnetic Reduced Graphene Oxide Nanocomposite: Synthesis, Characterization, and Application for High-Efficiency Detoxification of Aflatoxin B1
作者：Zhang, Chushu;Zhou, Haixiang;Cao, Shining;Chen, Jing;Qu, Chunjuan;Tang, Yueyi;Wang, Mian;Zhu, Lifei;Zhang, Jiancheng;Liu, Xiaoyue
关键词：adsorbent; aflatoxin B-1; detoxification; food safety; magnetic reduced graphene oxide
-
The Effects of Soybean-Tea Intercropping on the Photosynthesis Activity of Tea Seedlings Based on Canopy Spectral, Transcriptome and Metabolome Analyses
作者：Li, Xiaojiang;Wang, Shuangshuang;Sun, Litao;Shen, Jiazhi;Xu, Xiuxiu;Ding, Zhaotang;Xu, Yang;Mao, Yilin;Wang, Yu
关键词：intercropping; tea seedlings; canopy spectral; photosynthesis activity; light harvesting; photosystem
-
Establishment of deep learning model for the growth of tea cutting seedlings based on hyperspectral imaging technique
作者：Li, He;Sun, Litao;Zaman, Shah;Shen, Jiazhi;Li, Xiaojiang;Ding, Zhaotang;Li, He;Mao, Yilin;Fan, Kai;Shen, Yaozong;Xu, Yang;Chen, Hao;Wang, Yu;Shi, Hongtao;Bi, Caihong
关键词：Tea cutting seedings; Hyperspectral imaging technique; Biomass; Nondestructive testing; Deep learning
-
Flavor Improvement of Maillard Reaction Intermediates Derived from Enzymatic Hydrolysates of Oudemansiella raphanipes Mushroom
作者：Hou, Furong;Song, Shasha;Cui, Wenjia;Gong, Zhiqing;Wang, Yansheng;Wang, Wenliang;Yu, Zipeng
关键词：Oudemansiella raphanipes; Maillard intermediates; enzymatic hydrolysate; flavor substances
-
Rapid monitoring of tea plants under cold stress based on UAV multi-sensor data
作者：Mao, Yilin;Li, He;Wang, Yu;Wang, Huan;Xu, Yang;Ding, Zhaotang;Fan, Kai;Shen, Jiazhi;Ding, Zhaotang;Ding, Shibo;Wang, Hui
关键词：Tea plant; Cold stress; UAV; Multi-source remote sensing; Deep learning
