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Salivary carbonic anhydrase II in winged aphid morph facilitates infection viruses

文献类型： 外文期刊

作者： Guo, Huijuan 1 ; Zhang, Yanjing 1 ; Li, Bingyu 1 ; Li, Chenwei 3 ; Shi, Qingyun 1 ; Zhu-Salzman, Keyan 4 ; Ge, Feng 5 ; Sun, Yucheng 1 ;

作者机构： 1.Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects &, Beijing 100101, Peoples R China

2.Univ Chinese Acad Sci, CAS Ctr Excellence Biot Interact, Beijing 100049, Peoples R China

3.Hebei Univ, Sch Life Sci, Baoding 071002, Peoples R China

4.Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA

5.Shandong Acad Agr Sci, Inst Plant Protect, Jinan 250100, Peoples R China

关键词： carbonic anhydrase; aphid; cell wall; vesicle trafficking; virus transmission

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：11.1； 五年影响因子：12.0 ）

ISSN： 0027-8424

年卷期： 2023 年 120 卷 14 期

页码：

收录情况： SCI

摘要： Aphids are the most common insect vector transmitting hundreds of plant viruses. Aphid wing dimorphism (winged vs. wingless) not only showcases the phenotypic plas-ticity but also impacts virus transmission; however, the superiority of winged aphids in virus transmission over the wingless morph is not well understood. Here, we show that plant viruses were efficiently transmitted and highly infectious when associated with the winged morph of Myzus persicae and that a salivary protein contributed to this difference. The carbonic anhydrase II (CA-II) gene was identified by RNA-seq of sali-vary glands to have higher expression in the winged morph. Aphids secreted CA-II into the apoplastic region of plant cells, leading to elevated accumulation of H+. Apoplastic acidification further increased the activities of polygalacturonases, the cell wall homo-galacturonan (HG)-modifying enzymes, promoting degradation of demethylesterified HGs. In response to apoplastic acidification, plants accelerated vesicle trafficking to enhance pectin transport and strengthen the cell wall, which also facilitated virus translocation from the endomembrane system to the apoplast. Secretion of a higher quantity of salivary CA-II by winged aphids promoted intercellular vesicle transport in the plant. The higher vesicle trafficking induced by winged aphids enhanced disper-sal of virus particles from infected cells to neighboring cells, thus resulting in higher virus infection in plants relative to the wingless morph. These findings imply that the difference in the expression of salivary CA-II between winged and wingless morphs is correlated with the vector role of aphids during the posttransmission infection process, which influences the outcome of plant endurance of virus infection.

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