文献类型： 外文期刊

作者： Leng, Xiangpeng ¹ ; Miao, Wenjun ³ ; Li, Jizhen ¹ ; Liu, Yuanxia ¹ ; Zhao, Wenwen ⁴ ; Mu, Qian ⁵ ; Li, Qiu ³ ;

作者机构： 1.Qingdao Agr Univ, Inst Grape Sci & Engn, Coll Hort, Engn Lab Genet Improvement Hort Crops Shandong Pro, Qingdao 266109, Peoples R China

2.Qingdao Agr Univ, Coll Hort, Lab Qual & Safety Risk Assessment Fruit Qingdao, MARA, Qingdao 266109, Peoples R China

3.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Peoples R China

4.Qingdao Univ, Sch Basic Med Sci, 308 Ningxiafrr Rd, Qingdao 266021, Shandong, Peoples R China

5.Shandong Acad Agr Sci, Shandong Acad Grape, Jinan 250110, Peoples R China

关键词： Grapevine; Polysaccharides; Structural analysis; Antioxidant and anti-inflammatory activities

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.1； 五年影响因子：7.7 ）

ISSN： 0963-9969

年卷期： 2023 年 163 卷

页码：

收录情况： SCI

摘要： In this study, four wine grape polysaccharides were extracted and optimized by using an efficient ultrasound -assisted extraction. A three-level, three-factor Box Behnken Design (BBD) combining with response surface methodology (RSM) was employed to optimize the extraction conditions including ultrasonic power, ultrasonic time and liquid-to-solid ratio. Furthermore, their physicochemical structures, antioxidant and liver protective activity were investigated and compared. Results revealed that the functional groups and monosaccharide compositions of these grape polysaccharides collected from different varieties were similar. Nevertheless, their molecular weights, molar ratios of monosaccharide compositions and surface morphological features were different. And the antioxidant activities of these polysaccharides were screened by free radical scavenging test. 'Beichun' (BC) and 'Benni fuji' (BF) polysaccharides possessed better antioxidant function. Further, the in vivo evaluation indicated that the polysaccharides of BC and BF have a protective effect against myocardial I/R injury in mice by inhibiting myocardial necroptosis mediated by mitochondrial ROS generation. Therefore, BC and BF grapes have potential applications in the medical and food industries.