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Serine/threonine Kinases Play Important Roles in Regulating Polyunsaturated Fatty Acid Biosynthesis in Synechocystis sp. PCC6803

文献类型： 外文期刊

作者： Chen, Gao 1 ; Cao, Yuelei 1 ; Zhong, Huairong 2 ; Wang, Xiaodong 2 ; Li, Yanle 2 ; Cui, Xiaoyan 1 ; Lu, Xiaoyuan 1 ; Bi, 1 ;

作者机构： 1.Shandong Normal Univ, Sch Life Sci, Jinan, Peoples R China

2.Shandong Acad Agr Sci, Biotechnol Res Ctr, Shandong Prov Key Lab Genet Improvement Ecol & Ph, Jinan, Peoples R China

3.Tianjin Agr Univ, Key Lab Aquat Ecol & Aquaculture Tianjin, Coll Fishery, Tianjin, Peoples R China

关键词： microalgae; serine; threonine kinase system; polyunsaturated fatty acids; biosynthesis; Synechocystis sp; PCC6803

期刊名称：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY （ 影响因子：5.89； 五年影响因子：5.973 ）

ISSN： 2296-4185

年卷期： 2021 年 9 卷

页码：

收录情况： SCI

摘要： Serine/threonine kinases (STKs) play important roles in prokaryotic cellular functions such as growth, differentiation, and secondary metabolism. When the external environment changes, prokaryotes rely on signal transduction systems, including STKs that quickly sense these changes and alter gene expression to induce the appropriate metabolic changes. In this study, we examined the roles of the STK genes spkD and spkG in fatty acid biosynthesis in the unicellular cyanobacterium Synechocystis sp. PCC6803, using targeted gene knockout. The linoleic acid (C18: 2), gamma-linolenic acid (C18: 3n6), alpha-linolenic acid (C18: 3n3), and stearidonic acid (C18: 4) levels were significantly lower in spkD and spkG gene knockout mutants than in the wild type at a culture temperature of 30 degrees C and a light intensity of 40 mu mol.m(-2).s(-1). The expression levels of fatty acid desaturases and STK genes differed between the spkD and spkG gene knockout mutants. These observations suggest that spkD and spkG may directly or indirectly affect the fatty acid composition in Synechocystis sp. PCC6803 by regulating the expression of fatty acid desaturases genes. Therefore, the STK genes spkD and spkG play important roles in polyunsaturated fatty acid biosynthesis in Synechocystis sp. PCC6803. These findings could facilitate the development of cyanobacteria germplasm resources that yield high levels of fatty acids. In addition, they provide a theoretical basis for the genetic engineering of cyanobacteria with improved yields of secondary metabolites and increased economic benefits.

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[1]Serine/threonine Kinases Play Important Roles in Regulating Polyunsaturated Fatty Acid Biosynthesis in Synechocystis sp. PCC6803. Chen, Gao,Cao, Yuelei,Cui, Xiaoyan,Lu, Xiaoyuan,Dai, Meixue,Chen, Gao,Cao, Yuelei,Zhong, Huairong,Wang, Xiaodong,Li, Yanle,Cui, Xiaoyan,Lu, Xiaoyuan,Bi, Xiangdong. 2021

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