文献类型： 外文期刊
作者： Yang, Yuan-Xue 1 ; Lin, Rong-Hua 2 ; Li, Zhuo 1 ; Wang, Ai-Yu 1 ; Xue, Chao 1 ; Duan, Ai-Ling 1 ; Zhao, Ming 1 ; Zhang, J 1 ;
作者机构： 1.Shandong Acad Agr Sci, Cotton Res Ctr, Jinan, Peoples R China
2.Minist Agr & Rural Affairs, Inst Control Agrochem, Beijing, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Peoples R China
关键词： Aphis craccivora; P450 genes; GST genes; imidacloprid; expression induction; RNA interference
期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.566； 五年影响因子：4.804 ）
ISSN： 1664-042X
年卷期： 2021 年 11 卷
页码：
收录情况： SCI
摘要： Aphis craccivora (Koch) is an economically important pest that affects legumes in worldwide. Chemical control is still the primary efficient method for A. craccivora management. However, the mechanism underlying insecticide resistance in A. craccivora has not been elucidated. A previous study observed that piperonyl butoxide (PBO) and diethyl maleate (DEM) significantly synergized imidacloprid in A. craccivora field populations, indicating that cytochrome P450 (P450) and glutathione S-transferase (GST) genes may play pivotal roles in imidacloprid resistance. In this study, 38 P450 genes and 10 GST genes were identified in A. craccivora through transcriptomic analysis. The expression levels of these P450 and GST genes were measured in susceptible (SUS) strains of A. craccivora under imidacloprid treatment with LC15, LC50, and LC85 doses. The expression levels of CYP18A1, CYP6CY21, CYP6DA1, CYP6DA2, CYP4CJ1, CYP4CJ2, and CYP380C6 were up-regulated in the three treatments. Most of these genes belong to CYP3 and CYP4 Clans. In addition, the expression levels of all P450 and GST genes in A. craccivora were also measured in the Juye (JY) and Linqing (LQ) field populations. The expression levels of CYP6DA2, CYP4CJ1, and CYP380C6 were up-regulated in the SUS strain after imidacloprid treatment at three doses, and these genes were overexpressed in the JY population. Furthermore, the sensitivity of A. craccivora to imidacloprid was significantly increased after knockdown of CYP380C6 and CYP6DA2 through RNA interference. These results may help to elucidate the mechanisms underlying of imidacloprid resistance in A. craccivora.
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