文献类型： 外文期刊

作者： Peng, X. ¹ ; Qu, M. J. ² ; Wang, S. J. ¹ ; Huang, Y. X. ¹ ; Chen, C. ¹ ; Chen, M. H. ¹ ;

作者机构： 1.Northwest A&F Univ, Key Lab Crop Pest Integrated Pest Management Loes, State Key Lab Crop Stress Biol Arid Areas, Minist Agr & Rural Affairs, Yangling, Shaanxi, Peoples R China

2.Shandong Acad Agr Sci, Shandong Peanut Res Inst, Qingdao, Peoples R China

关键词： molecular docking; beta‐ cypermethrin; imidacloprid; survival rate; chemosensory proteins; bird cherry‐ oat aphid

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）

ISSN： 0962-1075

年卷期： 2021 年 30 卷 2 期

页码：

收录情况： SCI

摘要： Rhopalosiphum padi is a worldwide agricultural pest. Chemosensory proteins (CSPs) are considered to be a type of transporters which can bind chemicals from external environments. Previous research showed that the expression of some insect CSPs were significantly increased after exposure to insecticides, and CSPs were involved in insecticide resistance or susceptibility. However, the role of CSPs in the susceptibility and response of R. padi to insecticides is still unknown. In this study, we identified eight CSP (RpCSP) from R. padi by genome-wide investigation. Seven RpCSP genes had two exons, while RpCSP7 had three exons. qPCR analyses showed that the mRNA levels of the eight RpCSP genes were significantly affected by imidacloprid and beta-cypermethrin in different post-treatment periods. Molecular docking predicted that there were hydrogen bonding sites which played key roles in binding of RpCSP4, RpCSP5, RpCSP6, RpCSP7 and RpCSP10 with imidacloprid and beta-cypermethrin. Knockdown of RpCSP4, RpCSP5, RpCSP6 and RpCSP10 by RNA interference significantly increased the aphid mortality under two sublethal concentrations of imidacloprid. Mortalities under two sublethal concentrations of beta-cypermethrin conditions were significantly higher after injection of R. padi with dsCSP4 and dsCSP6. The results indicate that some RpCSP genes are involved in the insecticide susceptibility of R. padi.