文献类型： 外文期刊

作者： Li, Hanwen ¹ ; Nian, Jinqiang ¹ ; Fang, Shuang ¹ ; Guo, Meng ¹ ; Huang, Xiahe ³ ; Zhang, Fengxia ¹ ; Wang, Qing ¹ ; Zhang, Jian ¹ ; Bai, Jiaoteng ¹ ; Dong, Guojun ⁵ ; Xin, Peiyong ¹ ; Xie, Xianzhi ⁶ ; Chen, Fan ² ; Wang, Guodong ¹ ; Wang, Yingchun ² ; Qian, Qian ⁵ ; Zuo, Jianru ¹ ; Chu, Jinfang ¹ ; Ma, Xiaohui ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, State Key Lab Plant Genom, Beijing 100101, Peoples R China

2.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China

3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Laboratoty Mol Dev Biol, Beijing 100101, Peoples R China

4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China

5.Chinese Acad Agr Sci, China Natl Rice Res Inst, State Key Laboratoty Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China

6.Shandong Acad Agr Sci, Inst Wetland Agr & Ecol, Jinan 250100, Shandong, Peoples R China

7.Hainan Yazhou Bay Lab, Sanya 572025, Hainan, Peoples R China

8.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Beijing 100101, Peoples R China

关键词： Rice; Stringent response; Nitrogen starvation response; (p)ppGpp; ARE2

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：5.723； 五年影响因子：5.755 ）

ISSN： 1673-8527

年卷期： 2022 年 49 卷 5 期

页码：

收录情况： SCI

摘要： Nitrogen is an essential macronutrient for all living organisms and is critical for crop productivity and quality. In higher plants, inorganic nitrogen is absorbed through roots and then assimilated into amino acids by the highly conserved glutamine synthetase/glutamine:2-oxoglutarate aminotransferase (GS/GOGAT) cycle. How nitrogen metabolism and nitrogen starvation responses of plants are regulated remains largely unknown. Previous studies revealed that mutations in the rice ABNORMAL CYTOKININ RESPONSE1 (ABC1) gene encoding Fd-GOGAT cause a typical nitrogen deficiency syndrome. Here, we show that ARE2 (for ABC1 REPRESSOR2) is a key regulator of nitrogen starvation responses in rice. The are2 mutations partially rescue the nitrogen-deficient phenotype of abc1 and the are2 mutants show enhanced tolerance to nitrogen deficiency, suggesting that ARE2 genetically interacts with ABC1/Fd-GOGAT. ARE2 encodes a chloroplast-localized RelA/SpoT homolog protein that catalyzes the hydrolysis of guanosine pentaphosphate or tetraphosphate (p)ppGpp, an alarmone regulating the stringent response in bacteria under nutritional stress conditions. The are2 mutants accumulate excessive amounts of (p)ppGpp, which correlate with lower levels of photosynthetic proteins and higher amino acid levels. Collectively, these observations suggest that the alarmone (p)ppGpp mediates nitrogen stress responses and may constitute a highly conserved mechanism from bacteria to plants. Copyright (C) 2022, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.