文献类型： 外文期刊
作者： Wang, Chengpeng 1 ; Li, Yang 2 ; Wang, Na 2 ; Yu, Qin 2 ; Li, Yonghong 3 ; Gao, Junping 2 ; Zhou, Xiaofeng 2 ; Ma, Nan 2 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Leisure Agr, Key Lab East China Urban Agr, Minist Agr & Rural Affairs, Jinan 250100, Peoples R China
2.China Agr Univ, Dept Ornamental Hort, Beijing Key Lab Dev & Qual Control Ornamental Crop, Beijing 100193, Peoples R China
3.Shenzhen Polytech, Sch Appl Chem & Biotechnol, Shenzhen 518038, Peoples R China
关键词： CRISPR; CAS9; gene editing; RhEIN2; rose
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）
ISSN： 1672-9072
年卷期： 2023 年 65 卷 4 期
页码：
收录情况： SCI
摘要： The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-related nuclease 9 (Cas9) system enables precise, simple editing of genes in many animals and plants. However, this system has not been applied to rose (Rosa hybrida) due to the genomic complexity and lack of an efficient transformation technology for this plant. Here, we established a platform for screening single-guide RNAs (sgRNAs) with high editing efficiency for CRISPR/Cas9-mediated gene editing in rose using suspension cells. We used the Arabidopsis thaliana U6-29 promoter, which showed high activity for driving sgRNA expression, to modify the CRISPR/Cas9 system. We used our highly efficient optimized CRISPR/Cas9 system to successfully edit RhEIN2, encoding an indispensable component of the ethylene signaling pathway, resulting in ethylene insensitivity in rose. Our optimized CRISPR/Cas9 system provides a powerful toolbox for functional genomics, molecular breeding, and synthetic biology in rose.
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