文献类型： 外文期刊
作者： Wang, Chengpeng 1 ; Li, Yang 2 ; Wang, Na 2 ; Yu, Qin 2 ; Li, Yonghong 3 ; Gao, Junping 2 ; Zhou, Xiaofeng 2 ; Ma, Nan 2 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Leisure Agr, Key Lab East China Urban Agr, Minist Agr & Rural Affairs, Jinan 250100, Peoples R China
2.China Agr Univ, Dept Ornamental Hort, Beijing Key Lab Dev & Qual Control Ornamental Crop, Beijing 100193, Peoples R China
3.Shenzhen Polytech, Sch Appl Chem & Biotechnol, Shenzhen 518038, Peoples R China
关键词： CRISPR; CAS9; gene editing; RhEIN2; rose
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）
ISSN： 1672-9072
年卷期： 2023 年 65 卷 4 期
页码：
收录情况： SCI
摘要： The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-related nuclease 9 (Cas9) system enables precise, simple editing of genes in many animals and plants. However, this system has not been applied to rose (Rosa hybrida) due to the genomic complexity and lack of an efficient transformation technology for this plant. Here, we established a platform for screening single-guide RNAs (sgRNAs) with high editing efficiency for CRISPR/Cas9-mediated gene editing in rose using suspension cells. We used the Arabidopsis thaliana U6-29 promoter, which showed high activity for driving sgRNA expression, to modify the CRISPR/Cas9 system. We used our highly efficient optimized CRISPR/Cas9 system to successfully edit RhEIN2, encoding an indispensable component of the ethylene signaling pathway, resulting in ethylene insensitivity in rose. Our optimized CRISPR/Cas9 system provides a powerful toolbox for functional genomics, molecular breeding, and synthetic biology in rose.
- 相关文献
作者其他论文 更多>>
-
Lily LlHSFC2 coordinates with HSFAs to balance heat stress response and improve thermotolerance
作者：Wu, Ze;Li, Ting;Ding, Liping;Teng, Renda;Xu, Sujuan;Teng, Nianjun;Wu, Ze;Li, Ting;Ding, Liping;Teng, Renda;Xu, Sujuan;Teng, Nianjun;Wu, Ze;Wang, Chengpeng;Cao, Xing
关键词：AtHSFA2; heat stress transcription factor; high temperature; Lilium longiflorum; LlHSFA3A; LlHSFC2; thermotolerance
-
Small target tea bud detection based on improved YOLOv5 in complex background
作者：Wang, Mengjie;Meng, Hewei;Li, Yaping;Wang, Mengjie;Li, Yang;Chen, Zhiwei;Gui, Zhiyong;Dong, Chunwang
关键词：object detection; deep information extraction; lightweight; MPDIoU; YOLOv5; attention mechanism
-
Applications of CRISPR/Cas9 Technology in Ornamental Plants
作者：Liu, Hetong;Liu, Yun;Wei, Yamiao;Zheng, Chengshu;Liu, Hetong;Hu, Zongxia;Wu, Xiang;Wang, Chengpeng;Wang, Zhenzhen;Wang, Zhenzhen;Liu, Hetong;Liu, Yun;Wei, Yamiao;Zheng, Chengshu
关键词：CRISPR/Cas9; Ornamental plants; Applications
-
Moderation of nitrogen input and integration of legumes via intercropping enable sustainable intensification of wheat-maize double cropping in the North China Plain: A four-year rotation study
作者：Xia, Haiyong;Qiao, Yuetong;Li, Xiaojing;Xue, Yanhui;Wang, Na;Xia, Haiyong;Qiao, Yuetong;Li, Xiaojing;Xia, Haiyong;Yan, Wei;Xue, Yanfang;Cui, Zhenling;Cui, Zhenling;Xia, Haiyong;van der Werf, Wopke
关键词：Simultaneous intercropping; Land equivalent ratio; Gross margin; Sustainability and resilience; Temporal stability
-
Detection of anthocyanin content in fresh Zijuan tea leaves based on hyperspectral imaging
作者：Dai, Fushuang;Yang, Chongshan;Liu, Zhongyuan;An, Ting;Dong, Chunwang;Dai, Fushuang;Shi, Jiang;Yang, Chongshan;Li, Yang;Liu, Zhongyuan;An, Ting;Yan, Peng;Dai, Fushuang;Zhao, Yan;Li, Xiaoli
关键词：Hyperspectral imaging; Zijuan tea; Chemometric method; Anthocyanin content; Tenderness; Filtering of variables
-
A Tea Buds Counting Method Based on YOLOv5 and Kalman Filter Tracking Algorithm
作者：Li, Yang;Zhang, Rentian;Cheng, Yifan;Dong, Chunwang;Ma, Rong;Dong, Chunwang;Zhang, Rentian
关键词：
-
Neoagaro-oligosaccharides Improve the Postharvest Flower Quality and Vase Life of Cut Rose 'Gaoyuanhong'
作者：Wan, Yanling;Wen, Chao;Wang, Chengpeng;Wan, Yanling;Gong, Linfeng;Zeng, Hanting
关键词：abscisic acid; bacteria number; fresh cut flowers; preservatives; water balance
