文献类型： 外文期刊
作者： Wang, H. M. 1 ; Zhao, H. H. 1 ; Zhao, C. Z. 2 ; Chu, D. 1 ;
作者机构： 1.Qingdao Agr Univ, Coll Agron & Plant Protect, Key Lab Integrated Crop Pest Management Shandong, Qingdao 266109, Peoples R China
2.Shandong Acad Agr Sci, Biotech Res Ctr, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan 250100, Peoples R China
期刊名称：RUSSIAN JOURNAL OF GENETICS （ 影响因子：0.581； 五年影响因子：0.729 ）
ISSN： 1022-7954
年卷期： 2014 年 50 卷 10 期
页码：
收录情况： SCI
摘要： The soybean cyst nematode Heterodera glycines Ichinohe is a severe agricultural pest for which genetic resources are limited. In this study, 295 simple sequence repeats (SSRs) were identified from 259 expressed sequenced tags (ESTs), which were selected from 9,443 unigenes. The successful primer pairs were designed against six regions. In total, 30 alleles were identified from 30 individuals using the six markers, with an average of five alleles per locus (range, 4-7). The observed and expected heterozygosities were 0.074-0.900 and 0.266-0.775, respectively. Significant departure from Hardy-Weinberg equilibrium was found at three of the six loci. The EST-based SSR markers developed in this study may contribute to better under-standing of the genetic structure of H. glycines populations.
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