您好，欢迎访问山东省农业科学院文献资源数据库平台

Genetic diversity in Chinese sorghum landraces revealed by chloroplast simple sequence repeats

文献类型： 外文期刊

作者： Li, Ruyu 1 ; Zhang, Han 2 ; Zhou, Xincheng 3 ; Guan, Yanan 2 ; Yao, Fengxia 2 ; Song, Guoan 2 ; Wang, Jiancheng 2 ; Zhang, Chunqing 1 ;

作者机构： 1.Shandong Agr Univ, Natl Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

2.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Shandong, Peoples R China

3.Shandong Acad Agr Sci, Vegetable Res Inst, Jinan 250100, Shandong, Peoples R China

关键词： Chloroplast; Genetic diversity; Kaoliang; Microsatellite; Origin; Sorghum bicolor

期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：1.524； 五年影响因子：1.713 ）

ISSN： 0925-9864

年卷期： 2010 年 57 卷 1 期

页码：

收录情况： SCI

摘要： A collection of sorghum, including more than 12,000 Chinese landraces, has been constructed and maintained in China. However, the genetic diversity of Chinese sorghum landraces has not been fully investigated, and the origin of Chinese sorghum is still in dispute. In this study, the complete chloroplast genome sequence of sorghum line Tx623B was searched for simple sequence repeats (SSRs). 31 SSR loci with at least 10 mononucleotide repeats or five dinucleotide repeats were identified, and primer pairs for 27 loci were designed. Chloroplast DNA variation in cultivated sorghum was investigated by using these primer pairs on 185 Chinese sorghum landraces and 70 cultivated sorghum accessions from other countries. Among the 27 loci, 14 were polymorphic. The number of alleles per polymorphic locus ranged from 2 to 5 with an average of 2.79. Allelic data at 14 polymorphic loci were combined to give 12 haplotypes. The average allelic diversity index across the 14 polymorphic loci and corresponding haplotype diversity were markedly lower for Chinese sorghum landraces than were those for accessions from other countries. However, Chinese sorghum landraces shared a predominant allele at each polymorphic locus and a predominant haplotype with foreign accessions. Our results indicate that Chinese landraces experienced a severe maternal bottleneck during the introduction process with a predominant haplotype being present in 171 of 185 accessions analyzed. Except for one rare exception, haplotypes found in Chinese landraces were either identical or closely related to those found in foreign accessions and could not be separated clearly from them by cluster analysis. Our results are consistent with the hypothesis of African origin of Chinese sorghum.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台