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Genome-wide identification of PTI1 family in Setaria italica and salinity-responsive functional analysis of SiPTI1-5

文献类型： 外文期刊

作者： Huangfu, Yongguan 1 ; Pan, Jiaowen 2 ; Li, Zhen 2 ; Wang, Qingguo 2 ; Mastouri, Fatemeh 3 ; Li, Ying 1 ; Yang, Stephen 4 ;

作者机构： 1.Northeast Forestry Univ, Coll Life Sci, Minist Educ, Key Lab Saline Alkali Vegetat Ecol Restorat, Harbin 150040, Heilongjiang, Peoples R China

2.Shandong Acad Agr Sci, Jinan 250100, Shandong, Peoples R China

3.Bota Biosci, 325 Vassar St Suite 2a, Cambridge, MA 02139 USA

4.Inst Biosci & Biotechnol Res, 9600 Gudelsky Dr, Rockville, MD 20850 USA

5.Shandong Agr & Engn Univ, Jinan 250100, Shandong, Peoples R China

6.Shanghai Normal Univ, Coll Life Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China

7.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Shandong, Peoples R China

关键词： Foxtail millet (Setaria italica); Pto-interacting 1 genes (PTI1s); Expression pattern; Functional identification; Salt stress

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2021 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background PTI1 (Pto-interacting 1) protein kinase belongs to the receptor-like cytoplasmic kinase (RLCK) group of receptor-like protein kinases (RLK), but lack extracellular and transmembrane domains. PTI1 was first identified in tomato (Solanum lycopersicum) and named SlPTI1, which has been reported to interact with bacterial effector Pto, a serine/threonine protein kinase involved in plant resistance to bacterial disease. Briefly, the host PTI1 specifically recognizes and interacts with the bacterial effector AvrPto, which triggers hypersensitive cell death to inhibit the pathogen growth in the local infection site. Previous studies have demonstrated that PTI1 is associated with oxidative stress and hypersensitivity. Results We identified 12 putative PTI1 genes from the genome of foxtail millet (Setaria italica) in this study. Gene replication analysis indicated that both segmental replication events played an important role in the expansion of PTI1 gene family in foxtail millet. The PTI1 family members of model plants, i.e. S. italica, Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), maize (Zea mays), S. lycopersicum, and soybean (Glycine max), were classified into six major categories according to the phylogenetic analysis, among which the PTI1 family members in foxtail millet showed higher degree of homology with those of rice and maize. The analysis of a complete set of SiPTI1 genes/proteins including classification, chromosomal location, orthologous relationships and duplication. The tissue expression characteristics revealed that SiPTI1 genes are mainly expressed in stems and leaves. Experimental qRT-PCR results demonstrated that 12 SiPTI1 genes were induced by multiple stresses. Subcellular localization visualized that all of foxtail millet SiPTI1s were localized to the plasma membrane. Additionally, heterologous expression of SiPTI1-5 in yeast and E. coli enhanced their tolerance to salt stress. Conclusions Our results contribute to a more comprehensive understanding of the roles of PTI1 protein kinases and will be useful in prioritizing particular PTI1 for future functional validation studies in foxtail millet.

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