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Purification, Characterization, and Application for Preparation of Antioxidant Peptides of Extracellular Protease from Pseudoalteromonas sp. H2

文献类型： 外文期刊

作者： Liu, Dan 1 ; Huang, Jiafeng 1 ; Wu, Cuiling 3 ; Liu, Congling 1 ; Huang, Ran 1 ; Wang, Weng 1 ; Yin, Tingting 1 ; Yan, Xiaotao 1 ; He, Hailun 1 ; Chen, Leilei 4 ;

作者机构： 1.Cent S Univ, Sch Life Sci, Changsha 410013, Hunan, Peoples R China

2.Guangxi Med Univ, Dept Biochem & Mol Biol, Nanning 530021, Peoples R China

3.Changzhi Med Coll, Dept Biochem, Changzhi 046000, Peoples R China

4.BTBU, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China

5.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Jinan 250100, Shandong, Peoples R China

关键词： Pseudoalteromonas; protease; hydrolysis; collagen; antioxidant peptides

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期： 2019 年 24 卷 18 期

页码：

收录情况： SCI

摘要： The study reported on the isolation of a metalloprotease named EH2 from Pseudoalteromonas sp. H2. EH2 maintained more than 80% activity over a wide pH range of 5-10, and the stability was also nearly independent of pH. Over 65% activity was detected at a wide temperature range of 20-70 degrees C. The high stability of the protease in the presence of different surfactants and oxidizing agents was also observed. Moreover, we also investigated the antioxidant activities of the hydrolysates generated from porcine and salmon skin collagen by EH2. The results showed that salmon skin collagen hydrolysates demonstrated higher DPPH (1,1-diphenyl-2-picrylhydrazyl) (42.88% +/- 1.85) and hydroxyl radical (61.83% +/- 3.05) scavenging activity than porcine skin collagen. For oxygen radical absorbance capacity, the hydrolysates from porcine skin collagen had higher efficiency (7.72 +/- 0.13 mu molTE/mu mol). Even 1 nM mixed peptides could effectively reduce the levels of intracellular reactive oxygen species. The two types of substrates exerted the best antioxidant activity when hydrolyzed for 3 h. The hydrolysis time and type of substrate exerted important effects on the antioxidant properties of hydrolysates. The hydrolyzed peptides from meat collagens by proteases have good antioxidant activity, which may have implications for the potential application of marine proteases in the biocatalysis industry.

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[1]Purification, Characterization, and Application for Preparation of Antioxidant Peptides of Extracellular Protease from Pseudoalteromonas sp. H2. Liu, Dan,Huang, Jiafeng,Liu, Congling,Huang, Ran,Wang, Weng,Yin, Tingting,Yan, Xiaotao,He, Hailun,Liu, Dan,Wu, Cuiling,Chen, Leilei,Chen, Leilei. 2019

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