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Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.)

文献类型: 外文期刊

作者: Wang, Linhai 1 ; Li, Genying 2 ; Pena, Roberto J. 3 ; Xia, Xianchun 1 ; He, Zhonghu 1 ;

作者机构: 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

2.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Shandong, Peoples R China

3.Int Maize & Wheat Improvement Ctr CIMMYT, Mexico City 06600, DF, Mexico

4.Int Maize & Wheat Improvement Ctr CIMMYT, Beijing 100081, Peoples R China

关键词: Triticum aestivum;wheat;glutenins;loci;alleles;polymerase chain reaction;DNA primers;genetic markers

期刊名称:JOURNAL OF CEREAL SCIENCE ( 影响因子:3.616; 五年影响因子:3.891 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Low-molecular-weight glutenin subunits (LMW-GS) play a key role in determining the processing quality of the end-use products of common wheat. The objectives of this study were to identify genes at Glu-A3 locus, develop the STS markers, and establish multiplex PCR with the STS markers for Glu-A3 alleles. Gene-specific PCR primers were designed to amplify six near-isogenic lines (NILs) and Glenlea with different Glu-A3 alleles (a, b, c, d, e, f and g) defined by the protein electrophoretic mobility. Three Glu-A3 genes with complete coding sequence were cloned, designated as GluA3-1, GluA3-2 and GluA3-3, respectively. Seven dominant allele-specific STS (sequence tagged sites) markers were designed based on the SNPs (single nucleotide polymorphisms) among different allelic variants for the discrimination of the Glu-A3 protein alleles a, b, c, d, e, f and g. Four multiplex PCRs were established including Glu-A3b + Glu-A3f, Glu-A3d + Glu-A3f, Glu-A3d + Glu-A3g, and Glu-A3b + Glu-A3e. These markers and multiplex-PCR systems were validated on 141 CIMMYT wheat varieties and advanced lines with different Glu-A3 alleles, confirming that they can be efficiently used in marker-assisted breeding.

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