文献类型： 外文期刊

作者： Gao, Xu ¹ ; Han, Jina ³ ; Zhu, Lixian ¹ ; Nychas, George-John E. ¹ ; Mao, Yanwei ¹ ; Yang, Xiaoyin ¹ ; Liu, Yunge ¹ ; Jiang, Xueqing ¹ ; Zhang, Yimin ¹ ; Dong, Pengcheng ¹ ;

作者机构： 1.Shandong Agr Univ, Lab Beef Proc & Qual Control, Tai An 271018, Peoples R China

2.Int Joint Res Lab China & Greece Digital Transform, Tai An 271018, Peoples R China

3.Shandong Acad Agr Sci, Poultry Inst, Poultry Breeding Engn Technol Ctr Shandong Prov, Shandong Prov Key Lab Poultry Dis Diag & Immunol, Jinan 250023, Peoples R China

4.Agr Univ Athens, Dept Food Sci & Human Nutr, Athens 11855, Greece

关键词： Salmonella typhimurium; two-component regulatory system; acid tolerance response; cross-protection; cationic antimicrobial peptide resistance mechanisms

期刊名称：FOODS （ 影响因子：5.2； 五年影响因子：5.5 ）

ISSN：

年卷期： 2024 年 13 卷 10 期

页码：

收录情况： SCI

摘要： Acidic stress in beef cattle slaughtering abattoirs can induce the acid adaptation response of in-plant contaminated Salmonella. This may further lead to multiple resistance responses threatening public health. Therefore, the acid, heat, osmotic and antibiotic resistances of Salmonella typhimurium (ATCC14028) were evaluated after a 90 min adaption in a pH = 5.4 "mild acid" Luria-Bertani medium. Differences in such resistances were also determined between the triangle phoP mutant and wild-type Salmonella strains to confirm the contribution of the PhoP/PhoQ system. The transcriptomic differences between the acid-adapted and triangle phoP strain were compared to explore the role of the PhoP/Q two-component system in regulating multi-stress resistance. Acid adaptation was found to increase the viability of Salmonella to lethal acid, heat and hyperosmotic treatments. In particular, acid adaptation significantly increased the resistance of Salmonella typhimurium to Polymyxin B, and such resistance can last for 21 days when the adapted strain was stored in meat extract medium at 4 degrees C. Transcriptomics analysis revealed 178 up-regulated and 274 down-regulated genes in the triangle phoP strain. The Salmonella infection, cationic antimicrobial peptide (CAMP) resistance, quorum sensing and two-component system pathways were down-regulated, while the bacterial tricarboxylic acid cycle pathways were up-regulated. Transcriptomics and RT-qPCR analyses revealed that the deletion of the phoP gene resulted in the down-regulation of the expression of genes related to lipid A modification and efflux pumps. These changes in the gene expression result in the change in net negative charge and the mobility of the cell membrane, resulting in enhanced CAMP resistance. The confirmation of multiple stress resistance under acid adaptation and the transcriptomic study in the current study may provide valuable information for the control of multiple stress resistance and meat safety.