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Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering

文献类型： 外文期刊

作者： Song, Mei-Fang 1 ; Zhang, Shu 2 ; Hou, Pei 3 ; Shang, Hong-Zhong 1 ; Gu, Hai-Ke 1 ; Li, Jing-Juan 2 ; Xiao, Yang 4 ; Guo, Lin 3 ; Su, Liang 3 ; Gao, Jian-Wei 2 ; Yang, Jian-Ping 3 ;

作者机构： 1.Beijing Radiat Ctr, Beijing 100875, Peoples R China

2.Shandong Acad Agr Sci, Inst Vegetables & Flowers, Shandong Key Lab Greenhouse Vegetable Biol, Jinan 250100, Peoples R China

3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China

4.Chinese Acad Ag

关键词： Arabidopsis thaliana; Chinese cabbage; Ectopic expression; De-etiolation; Flowering time; Phytochrome B

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN： 0167-4412

年卷期： 2015 年 87 卷 6 期

页码：

收录情况： SCI

摘要： Phytochrome B (phyB) is an essential red light receptor that predominantly mediates seedling de-etiolation, shade-avoidance response, and flowering time. In this study, we isolate a full-length cDNA of PHYB, designated BrPHYB, from Chinese cabbage (Brassica rapa L. ssp. pekinensis), and we find that BrphyB protein has high amino acid sequence similarity and the closest evolutionary relationship to Arabidopsis thaliana phyB (i.e., AtphyB). Quantitative reverse transcription (RT)-PCR results indicate that the BrPHYB gene is ubiquitously expressed in different tissues under all light conditions. Constitutive expression of the BrPHYB gene in A. thaliana significantly enhances seedling de-etiolation under red- and white-light conditions, and causes dwarf stature in mature plants. Unexpectedly, overexpression of BrPHYB in transgenic A. thaliana resulted in reduced expression of gibberellins biosynthesis genes and delayed flowering under short-day conditions, whereas AtPHYB overexpression caused enhanced expression of FLOWERING LOCUS T and earlier flowering. Our results suggest that BrphyB might play an important role in regulating the development of Chinese cabbage. BrphyB and AtphyB have conserved functions during de-etiolation and vegetative plant growth and divergent functions in the regulation of flowering time.

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