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Increased glycine betaine synthesis and salinity tolerance in AhCMO transgenic cotton lines

文献类型： 外文期刊

作者： Zhang, Huijun 2 ; Dong, Hezhong 1 ; Li, Weijiang 1 ; Sun, Yi 3 ; Chen, Shouyi 4 ; Kong, Xiangqiang 1 ;

作者机构： 1.Shandong Acad Agr Sci, Shandong Key Lab Cotton Culture & Physiol, Cotton Res Ctr, Jinan 250100, Peoples R China

2.Shanxi Acad Agr Sci, Cotton Res Inst, Yucheng 044000, Peoples R China

3.Agr Biotechnol Res Ctr Shanxi Prov, Taiyuan 030031, Peoples R China

4.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

关键词： salinity tolerance

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

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收录情况： SCI

摘要： Glycine betaine is an osmoprotectant that plays an important role and accumulates rapidly in many plants during salinity or drought stress. Choline monooxygenase (CMO) is a major catalyst in the synthesis of glycine betaine. In our previous study, a CMO gene (AhCMO) cloned from Atriplex hortensis was introduced into cotton (Gossypium hirsutum L.) via Agrobacterium mediation to enhance resistance to salinity stress. However, there is little or no knowledge of the salinity tolerance of the transgenic plants, particularly under saline-field conditions. In the present study, two transgenic AhCMO cotton lines of the T-3 generation were used to study the AhCMO gene expression, and to determine their salinity tolerance in both greenhouse and field under salinity stress. Molecular analysis confirmed that the transgenic plants expressed the AhCMO gene. Greenhouse study showed that on average, seedlings of the transgenic lines accumulated 26 and 131% more glycine betaine than those of non-transgenic plants (SM3) under normal and salt-stress (150 mmol l(-1) NaCl) conditions, respectively. The osmotic potential, electrolyte leakage and malondialdehyde (MDA) accumulation were significantly lower in leaves of the transgenic lines than in those of SM3 after salt stress. The net photosynthesis rate and Fv/Fm in transgenic cotton leaves were less affected by salinity than in non-transgenic cotton leaves. Therefore, transgenic cotton over-expressing AhCMO was more tolerant to salt stress due to elevated accumulation of glycine betaine, which provided greater protection of the cell membrane and photosynthetic capacity than in non-transgenic cotton. The seed cotton yield of the transgenic plants was lower under normal conditions, but was significantly higher than that of non-transgenic plants under salt-stressed field conditions. The results indicate that over-expression of AhCMO in cotton enhanced salt stress tolerance, which is of great value in cotton production in the saline fields.

  • 相关文献

[1]Genetic improvement of cotton tolerance to salinity stress. Ma, Xinrong,Dong, Hezhong,Li, Weijiang,Ma, Xinrong. 2011

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