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Isolation of nematophagous fungi from eggs and females of Meloidogyne spp. and evaluation of their biological control potential

文献类型: 外文期刊

作者: Aminuzzaman, F. M. 1 ; Xie, H. Y. 2 ; Duan, W. J. 3 ; Sun, B. D. 4 ; Liu, X. Z. 4 ;

作者机构: 1.Sher E Bangla Agr Univ, Fac Agr, Dept Plant Pathol, Dhaka, Bangladesh

2.Shandong Acad Agr Sci, High Tech Res Ctr, Jinan, Peoples R China

3.Tech Ctr Peoples Republ China, Ningbo Entry Exit Inspect & Quarantine Bur, Ningbo, Zhejiang, Peoples R China

4.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing, Peoples R China

关键词: Paecilomyces lilacinus; Pochonia chlamydosporia; Meloidogyne incognita; nematophagous fungi; screening; pellet formulation

期刊名称:BIOCONTROL SCIENCE AND TECHNOLOGY ( 影响因子:1.665; 五年影响因子:1.616 )

ISSN: 0958-3157

年卷期: 2013 年 23 卷 2 期

页码:

收录情况: SCI

摘要: Fungi were isolated from Meloidogyne spp. eggs and females on 102 field-collected root samples in China. Of the 235 fungi isolated (representing 18 genera and 26 species), the predominant fungi were Fusarium spp. (42.1% of the isolates collected), Fusarium oxysporum (13.2%), Paecilomyces lilacinus (12.8%), and Pochonia chlamydosporia (8.5%). The isolates were screened for their ability to parasitise Meloidogyne incognita eggs in 24-well tissue culture plates in two different tests. The percentage of eggs parasitised by the fungi, the numbers of unhatched eggs and alive and dead juveniles were counted at 4 and 7 days after inoculation. The most promising fungi included five Paecilomyces isolates, 10 Fusarium isolates, 10 Pochonia isolates and one Acremonium isolate in test 1 or test 2. Paecilomyces lilacinus YES-2 and P. chlamydosporia HDZ-9 selected from the in vitro tests were formulated in alginate pellets and evaluated for M. incognita control on tomato in a greenhouse by adding them into a soil with sand mixture at rates of 0.2, 0.4, 0.8 and 1.6% (w/w). P. lilacinus pellets at the highest rate (1.6%) reduced root galling by 66.7%. P. chlamydosporia pellets at the highest rate reduced the final nematode density by 90%. The results indicate that P. lilacinus and P. chlamydosporia as pellet formulation can effectively control root-knot nematodes.

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