‘孔府酥脆’枣试管苗离体叶片不定梢诱导和再生

孙清荣; 孙洪雁; 周广芳; 《植物资源与环境学报 》 2011 CSCD

摘要：Using in vitro leaves of Zizyphus jujuba ‘Kongfusucui’ plantlet as explants,effects of culture method,phytohormone proportion and culture time on induction rate of adventitious shoot from leaves and effects of sucrose concentrations in rooting media on rooting of adventitious shoot were studied.The results show that induction rate of adventitious shoot by two-step culture method(firstly cultured on medium Ⅰ for four weeks then cultured on medium Ⅱ for three weeks) is significantly higher than that by one-step culture method(cultured continuously on medium Ⅰ for seven weeks).Phytohormone proportion in medium Ⅰ has an obviously influence on induction rate of adventitious shoot,which gradually increases with increasing of TDZ concentration in medium Ⅰ.And adding 1.0 mg·L-1 TDZ in medium Ⅰ leads to induction rate with above 80%.With culture time prolonging(cultured for 1,2,3 or 4 weeks on medium Ⅰ,respectively),induction rate increases gradually.Sucrose concentration has a significant effect on rooting of adventitious shoot,sucrose with higher concentration(30 g·L-1) is beneficial to rooting.According to induction rate and growth status of adventitious shoot,it is determined that optimal culture method of adventitious shoot induction from Z.jujuba 'Kongfusucui' leaves is two-step culture method,that is,leaves firstly cultured on WPM medium containing 1.0 mg·L-1 TDZ and 0.5 mg·L-1 IAA for four weeks,and then cultured on WPM medium containing 0.5 mg·L-1 IAA and 1.0 mg·L-1 GA3 till adventitious shoot produced.

关键词： ‘孔府酥脆’枣 离体叶片 不定梢诱导 植株再生 两步培养法

苹果叶片不定梢诱导及起源的细胞组织学观察

孙清荣; 孙洪雁; 石荫坪; 敖光明; 《农业生物技术学报 》 2002 CSCD

摘要：本实验获得了苹果“世界一”、“北海道9号”、“千秋”等18个品种叶片的再生不定梢,研究了基因型、基本培养基、激素、暗培养条件等影响苹果叶片不定梢再生的因素。结果表明,基因型是影响叶片不定梢再生的关键因素。对不定梢起源进行细胞组织学探讨,发现苹果叶片不定梢起源多种多样,既有单细胞起源,又有多细胞起源;既有表皮细胞,又有叶肉细胞;既有维管束薄壁组织,又有叶柄基本组织。

关键词： 苹果 叶片 不定梢诱导 不定梢起源

莱阳矮樱桃成熟胚子叶不定梢诱导研究

孙清荣; 孙洪雁; 石荫坪; 杨建明; 《山东农业大学学报(自然科学版) 》 2000 北大核心 CSCD

关键词： 莱阳矮樱桃 成熟胚子叶 不定梢诱导